12263 Qiafilter Plasmid Maxi Kit (25) Lab Use - Application: Laboratory

QIAGEN QIAfilter Plasmid Maxi Kit (25): is designed for the large-scale isolation of high-yield high-quality plasmid DNA from bacterial cultures. It is ideal for researchers who need substantial amounts of purified plasmid DNA such as for transfection protein expression sequencing or other downstream applications.
Key Features:
High Yield : The kit is optimized for isolating up to 1 mg of plasmid DNA from bacterial cultures ranging from 500 mL to 1 liter. This high yield is ideal for large-scale experiments or industrial applications.
QIAfilter Filtration Technology : The QIAfilter technology simplifies the process by allowing for the efficient lysis of bacterial cells and filtration to remove cellular debris genomic DNA and other contaminants. This results in high-purity plasmid DNA suitable for sensitive applications.
High Purity : The purified plasmid DNA is free of contaminants such as endotoxins RNA and proteins which ensures that the plasmid DNA is of high quality for demanding downstream applications.
Scalability : This kit is designed for large-scale plasmid preparation ideal for producing large quantities of DNA for applications like transfection cloning sequencing and protein production.
Streamlined Protocol : The QIAfilter system significantly reduces the time and labor involved in plasmid isolation by simplifying the traditional alkaline lysis method. It also minimizes the need for multiple centrifugation and filtration steps.

Components:

QIAfilter Cartridge : This specialized filtration device helps to lyse bacterial cells and efficiently remove large debris resulting in a cleaner plasmid DNA preparation.
Buffers : The kit includes buffers for resuspension lysis neutralization washing and elution of plasmid DNA. These buffers are optimized for high yield and purity.
Spin Columns : The plasmid DNA binds to a silica membrane in the spin column where it is washed to remove impurities before being eluted.
Elution Solution : A buffer or water to elute the purified plasmid DNA from the column.

Benefits:

Efficient and High-Quality Plasmid Preparation : The filtration system allows for high-efficiency lysis and removal of contaminants ensuring that the plasmid DNA is highly purified and suitable for transfection and sequencing.
Time-Saving : By reducing the number of centrifugation and filtration steps the QIAfilter technology accelerates the plasmid preparation process making it more efficient than traditional methods.
Large-Scale Capacity : Suitable for large-scale plasmid preparations enabling researchers to obtain substantial amounts of plasmid DNA from large bacterial cultures.
Versatility : The kit works well with a variety of bacterial strains and plasmid sizes ensuring wide applicability.

Applications :
Transfection : The high-purity plasmid DNA is suitable for various transfection protocols including viral vector production and mammalian cell transfection.

Protein Production : Ideal for recombinant protein expression in bacterial or eukaryotic systems as it provides large amounts of plasmid DNA for protein expression studies.

Sequencing : The purified plasmid DNA is of sufficient quality for sequencing applications including Sanger sequencing.
Cloning : Perfect for cloning applications where high yields of plasmid DNA are required for further manipulation or construction of libraries.

The QIAfilter Plasmid Maxi Kit (25) : is a comprehensive and efficient solution for large-scale plasmid DNA preparation.